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PET酶

PETase
识别码
EC编号 3.1.1.101
数据库
IntEnz IntEnz浏览
BRENDA英语BRENDA BRENDA入口
ExPASy英语ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc英语MetaCyc 代谢路径
PRIAM英语PRIAM_enzyme-specific_profiles 概述
PDB RCSB PDB PDBj PDBe PDBsum

PET酶(PET水解酶,PETase)是由2016年在日本堺市的一处垃圾掩埋场之中所发现的大阪堺菌Ideonella sakaiensis)制造出来的一种水解酶[1]PET酶能将PET塑胶降解为其单体单-2-羟乙基对苯二甲酸(MHET)分子。MHET在细菌体内会进一步被MHET酶(MHETase)降解为羟乙基对苯二甲酸,接着在水中分解为对环境友善的对苯二甲酸乙二醇,并且可为其他细菌利用,最终产生二氧化碳与水。[2]

PET酶催化的反应式可简单表示为:[3]

(PET)n + H2O ⇌ (ethylene terephthalate)n-1 + MHET
一个带有两处氨基酸突变(R103GS131A)的PET酶表面图像,HEMT并接上了其活性位置。HEMT,1-(2-羟乙基) 4-甲基对苯二甲酸,是结构类似于MHET的物质,带有多一处的甲醇酯化。PDBID: 5XH3

直至2018年,已经有数种立体结构被解析出来,包括5XH3页面存档备份,存于互联网档案馆)、5XH2页面存档备份,存于互联网档案馆)、5XG0页面存档备份,存于互联网档案馆)、5XFZ页面存档备份,存于互联网档案馆)、5XFY页面存档备份,存于互联网档案馆)、5YNS页面存档备份,存于互联网档案馆) 、5XJH页面存档备份,存于互联网档案馆)等。

参考文献

  1. ^ Tanasupawat, Somboon; Takehana, Toshihiko; Yoshida, Shosuke; Hiraga, Kazumi; Oda, Kohei. Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate). International Journal of Systematic and Evolutionary Microbiology. August 2016, 66 (8): 2813–2818. ISSN 1466-5034. PMID 27045688. doi:10.1099/ijsem.0.001058. 
  2. ^ Yoshida, Shosuke; Hiraga, Kazumi; Takehana, Toshihiko; Taniguchi, Ikuo; Yamaji, Hironao; Maeda, Yasuhito; Toyohara, Kiyotsuna; Miyamoto, Kenji; Kimura, Yoshiharu. A bacterium that degrades and assimilates poly(ethylene terephthalate). Science. 2016-03-11, 351 (6278): 1196–1199 [2018-04-22]. ISSN 0036-8075. PMID 26965627. doi:10.1126/science.aad6359. (原始内容存档于2018-04-18). 简明摘要 (PDF) (2016-03-30) (英语). 
  3. ^ BRENDA - Information on EC 3.1.1.101 - poly(ethylene terephthalate) hydrolase. [2018-04-17]. (原始内容存档于2019-02-17). 

参见

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PET酶
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