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互补脱氧核糖核酸

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从在测试中使用cDNA DNA微阵列的输出。

互补DNA(英语:complementary DNA缩写cDNA)是一种利用逆转录酶,以RNA(通常是mRNA)为模板做成的复制品,经常用来将真核生物基因(以mRNA形式)复制到原核生物细胞中。
一个cDNA可含有一个或多个mRNA,含有许多来自不同基因的mRNA的cDNA称为cDNA基因库(cDNA library)
cDNA克隆的关键环节是以mRNA为模板利用逆转录酶合成cDNA。

用途

逆转录PCR过程中,RNA单链会被逆转录cDNA之后通过PCR进行复制。

参考文献

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互补脱氧核糖核酸
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