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PET酶

PETase
识别码
EC編號 3.1.1.101
数据库
IntEnz IntEnz浏览
BRENDA英语BRENDA BRENDA入口
ExPASy英语ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc英语MetaCyc 代谢路径
PRIAM英语PRIAM_enzyme-specific_profiles 概述
PDB RCSB PDB PDBj PDBe PDBsum

PET酶(PET水解酶,PETase)是由2016年在日本堺市的一處垃圾掩埋場之中所發現的大阪堺菌Ideonella sakaiensis)制造出来的一种水解酶[1]PET酶能將PET塑膠降解為其單體單-2-羥乙基對苯二甲酸(MHET)分子。MHET在細菌體內會進一步被MHET酶(MHETase)降解為羥乙基對苯二甲酸,接著在水中分解為對環境友善的對苯二甲酸乙二醇,並且可為其他細菌利用,最終產生二氧化碳與水。[2]

PET酶催化的反應式可簡單表示為:[3]

(PET)n + H2O ⇌ (ethylene terephthalate)n-1 + MHET
一個帶有兩處胺基酸突變(R103GS131A)的PET酶表面圖像,HEMT並接上了其活性位置。HEMT,1-(2-羥乙基) 4-甲基對苯二甲酸,是結構類似於MHET的物質,帶有多一處的甲醇酯化。PDBID: 5XH3

直至2018年,已經有數種立體結構被解析出來,包括5XH3页面存档备份,存于互联网档案馆)、5XH2页面存档备份,存于互联网档案馆)、5XG0页面存档备份,存于互联网档案馆)、5XFZ页面存档备份,存于互联网档案馆)、5XFY页面存档备份,存于互联网档案馆)、5YNS页面存档备份,存于互联网档案馆) 、5XJH页面存档备份,存于互联网档案馆)等。

参考文献

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  1. ^ Tanasupawat, Somboon; Takehana, Toshihiko; Yoshida, Shosuke; Hiraga, Kazumi; Oda, Kohei. Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate). International Journal of Systematic and Evolutionary Microbiology. August 2016, 66 (8): 2813–2818. ISSN 1466-5034. PMID 27045688. doi:10.1099/ijsem.0.001058. 
  2. ^ Yoshida, Shosuke; Hiraga, Kazumi; Takehana, Toshihiko; Taniguchi, Ikuo; Yamaji, Hironao; Maeda, Yasuhito; Toyohara, Kiyotsuna; Miyamoto, Kenji; Kimura, Yoshiharu. A bacterium that degrades and assimilates poly(ethylene terephthalate). Science. 2016-03-11, 351 (6278): 1196–1199 [2018-04-22]. ISSN 0036-8075. PMID 26965627. doi:10.1126/science.aad6359. (原始内容存档于2018-04-18). 简明摘要 (PDF) (2016-03-30) (英语). 
  3. ^ BRENDA - Information on EC 3.1.1.101 - poly(ethylene terephthalate) hydrolase. [2018-04-17]. (原始内容存档于2019-02-17). 

参见

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PET酶
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