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核糖核酸酶H

ribonuclease H
大腸桿菌E. coli)RNase HI的晶体结构[1]
识别码
EC編號 3.1.26.4
CAS号 9050-76-4
数据库
IntEnz IntEnz浏览
BRENDA英语BRENDA BRENDA入口
ExPASy英语ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc英语MetaCyc 代谢路径
PRIAM英语PRIAM_enzyme-specific_profiles 概述
PDB RCSB PDB PDBj PDBe PDBsum
基因本体 AmiGO / EGO
retroviral ribonuclease H
识别码
EC編號 3.1.26.13
数据库
IntEnz IntEnz浏览
BRENDA英语BRENDA BRENDA入口
ExPASy英语ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc英语MetaCyc 代谢路径
PRIAM英语PRIAM_enzyme-specific_profiles 概述
PDB RCSB PDB PDBj PDBe PDBsum

核糖核酸酶H(英語:Ribonuclease H,简写为RNase HRNH)是一类在DNA复制中催化DNA-RNA引物杂合体(如岡崎片段)的RNA部分的降解的核糖核酸酶。核糖核酸酶H可水解两个相邻RNA碱基之间的3'-磷酸键,但不能水解最后一个RNA核糖核酸与DNA的第一个脱氧核糖核酸之间的磷酸键,因此产生带5'端带一个核糖核酸的DNA链,之后由5'-3'核酸外切酶来切除。

H1、H2和H3三类RNase H的比较

细菌古菌真核生物都有两类RNase H,称为H1和H2,此外少数细菌还有一类H3[2]

参考文献

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  1. ^ PDB 1JL1; Goedken ER, Marqusee S. Native-state energetics of a thermostabilized variant of ribonuclease HI. Journal of Molecular Biology. December 2001, 314 (4): 863–71. PMID 11734003. doi:10.1006/jmbi.2001.5184. 
  2. ^ Cerritelli SM, Crouch RJ. Ribonuclease H: the enzymes in eukaryotes. The FEBS Journal. March 2009, 276 (6): 1494–505. PMC 2746905可免费查阅. PMID 19228196. doi:10.1111/j.1742-4658.2009.06908.x. 

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核糖核酸酶H
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