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T7 RNA聚合酶

T7 RNA聚合酶
T7RNA聚合酶(蓝),mRNA(淡蓝),双链DNA(橙)
标识
生物 T7噬菌体
符号 1
PDB 1MSW
UniProt P00573
其他数据

T7 RNA聚合酶(英语:T7 RNA Polymerase)是一种RNA聚合酶,分子量约99kDa。专门催化5'→3'方向的RNA形成过程。T7RNA聚合酶具有高度启动子专一性,且只会转录T7噬菌体中位于T7启动子下游的的DNA或DNA复制品。[1]

此酵素在合成RNA的过程中,需要DNA模板与离子(Mg2+)作为辅因子牛血清白蛋白(bovine serum albumin,BSA)及精胺(spermidine)对此酵素具促进效果。与细菌的RNA聚合酶的差异之一,在于T7RNA聚合酶不受抗生素利福平(rifampicin)抑制。[2]

参考文献

  1. ^ T7 RNA Polymerase (20 U/µL). www.thermofisher.com. [2023-01-22]. (原始内容存档于2022-12-04) (英语). 
  2. ^ Sastry, SS; Ross, BM. Nuclease activity of T7 RNA polymerase and the heterogeneity of transcription elongation complexes.. The Journal of biological chemistry. 1997-03-28, 272 (13): 8644–52. PMID 9079696. doi:10.1074/jbc.272.13.8644. 

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T7 RNA聚合酶
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