This is a list of airlines of Manitoba which have an air operator's certificate issued by Transport Canada, the country's civil aviation authority. These are airlines that are based in Manitoba.

Airline	Image	IATA	ICAO	Callsign	Hub airport(s) or headquarters	Notes
Calm Air		MO	CAV	CALM AIR	Thompson	Scheduled passenger service, charters, cargo[1]
Fast Air			PRB	POLAR BEAR	Winnipeg	Air ambulance services, charter, helicopters[2]
Harv's Air Service					Steinbach (South)	Charters, flight training, air taxi[3][4]
Keystone Air Service			KEE	KEYSTONE	Winnipeg/St. Andrews	Charters[5]
Kivalliq Air		FK			Winnipeg James Armstrong Richardson	Scheduled passenger service[6]
Missinippi Airways		MA		Missinippi	The Pas/Grace Lake	Charters, MEDIVAC (air ambulance), and scheduled services. Bases at Thompson, Norway House, Pukatawagan and Winnipeg.[7]
Perimeter Aviation		4B	PAG	PERIMETER	Winnipeg James Armstrong Richardson	Scheduled passenger service, charters, MEDIVAC (air ambulance), flight training[8]
SkyNorth Air					Winnipeg James Armstrong Richardson	Scheduled passenger service, charters[9]

Airline	Image	IATA	ICAO	Callsign	Hub airport(s) or headquarters	Notes
Canadian Airways					Winnipeg James Armstrong Richardson	1926 - 1941 Established as Western Canadian Airways, purchased by Canadian Pacific Airlines
First Nations Transportation					Gimli	2003 - 2009
Greyhound Air					Winnipeg James Armstrong Richardson	1996 - 1997
Lamb Air					The Pas	1934 - 1981
Transair		TZ	TTZ		Winnipeg James Armstrong Richardson	1947 - 1979 Started as Central Northern Airways, renamed Transair in 1956; sold to Pacific Western Airlines in 1979
Winnport					Winnipeg James Armstrong Richardson	1998 - 2002 Winnipeg-based air cargo company flying to China; renamed to Cargojet Airways
Air Manitoba		7N	NAM	MANITOBA[10]		Established as Ilford Airways in 1953, merged with Riverton Airways (c. 1960) to form Ilford Riverton Airways in 1963. Merged with Northland Airlines (c. 1961) to become Northland Air Manitoba in 1986 and renamed to Air Manitoba in 1992.Ceased passenger operations when AOC revoked in 1994. Existing as leasing company around 1997.[11]