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黄色荧光蛋白

黄色荧光蛋白
常见的CFP-YFP的FRET

黄色荧光蛋白(英语:Yellow Fluorescent Protein,缩写YFP)是一种绿色荧光蛋白(GFP)的变体[1],能发出黄色荧光(激发峰517nm,发射峰527nm)[2],从GFP变为YFP至少需要蛋白质序列上四个氨基酸残基(aa)发生改变,其中最重要的是203号位的苏氨酸变为酪氨酸(T203Y)[1],常作为青色荧光蛋白(CFP)荧光共振能量转移的受体。

参考文献

  1. ^ 1.0 1.1 Ormö, Mats; Cubitt, Andrew B.; Kallio, Karen; Gross, Larry A.; Tsien, Roger Y.; Remington, S. James. Crystal Structure of the Aequorea victoria Green Fluorescent Protein. Science. 1996-09-06, 273 (5280): 1392–1395. ISSN 0036-8075. PMID 8703075. doi:10.1126/science.273.5280.1392 (英语). 
  2. ^ Lambert, Talley. EYFP at FPbase. FPbase. [2021-02-22] (英语). 
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黄色荧光蛋白
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