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开放阅读框

样品序列显示三种不同的可能的阅读框。 起始密码子以紫色突出显示,而终止密码子以红色突出显示。

开放阅读框(英语:Open reading frame;缩写:ORF;其他译名:开放阅读框架开放读架等)是指在给定的阅读框架中,不包含终止密码子的一串序列。这段序列是生物个体的基因组中,可能作为蛋白质编码序列的部分。基因中的ORF包含并位于开始编码与终止编码之间。由于一段DNARNA序列有多种不同读取方式,因此可能同时存在许多不同的开放阅读框架。有一些计算机程序可分析出最有可能编码蛋白质的序列。

例子

一段5'-UCUAAAGGUCCA-3'序列。此序列共有3种读取法:

  1. UCU AAA GGU CCA
  2. CUA AAG GUC
  3. UAA AGG UCC

由于UAA为终止密码子,因此第三种读取法不具编译出蛋白质的潜力,故只有前两者为开放阅读框架。

找ORF的软件

在线寻找ORF的软件:ORF Finder页面存档备份,存于互联网档案馆

功能

ORF Finder 被用来预测已存在的编码区的小基因序列。也可用于大规模的开放式阅读框寻找。

参见

外部链接

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开放阅读框
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