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分子克隆

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基因复制
基因复制

分子克隆(英语:Molecular cloning)又译分子选殖分子纯化繁殖,指分离一个已知DNA序列,并以活体内方式获得许多复制品的过程。这一复制过程经常被用于增加并获取DNA片段中的基因,但因为这个方法本身使用了基因剪贴的技术,也可用来增加某些任意的DNA序列,如启动子、非编码序列、化学合成的寡核苷酸或是随机的DNA片段。

分子克隆目前多应用于基因克隆(英语:Gene cloning,又译基因选殖),是指由基因组或DNA中选取并分离单个基因,将此单个基因导入宿主细胞中,进行扩增而复制出许多相同拷贝的过程。

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分子克隆
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