Nucleic acid methods
Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.
Purification
[edit]- DNA extraction
- Phenol–chloroform extraction
- Minicolumn purification
- RNA extraction
- Boom method
- Synchronous coefficient of drag alteration (SCODA) DNA purification
Quantification
[edit]- Abundance in weight: spectroscopic nucleic acid quantitation
- Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
- High-throughput relative abundance: DNA microarray
- High-throughput absolute abundance: serial analysis of gene expression (SAGE)
- Size: gel electrophoresis
Synthesis
[edit]- De novo: oligonucleotide synthesis
- Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)
Kinetics
[edit]- Multi-parametric surface plasmon resonance[1][2]
- Dual-polarization interferometry[3]
- Quartz crystal microbalance with dissipation monitoring (QCM-D)[4]
Gene function
[edit]Other
[edit]- Bisulfite sequencing
- DNA sequencing
- Expression cloning
- Fluorescence in situ hybridization
- Lab-on-a-chip
- Comparison of nucleic acid simulation software
- Northern blot
- Nuclear run-on assay
- Radioactivity in the life sciences
- Southern blot
- Differential centrifugation (sucrose gradient)
- Toeprinting assay
- Several bioinformatics methods, as seen in list of RNA structure prediction software
See also
[edit]References
[edit]- ^ Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484.
- ^ Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9): 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116. S2CID 206070778.
- ^ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
- ^ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101.
- ^ Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281.
External links
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