The 2000 Richmond Spiders football team represented the University of Richmond during the 2000 NCAA Division I-AA football season. It was the program's 117th season and they finished as Atlantic 10 Conference (A-10) co-champions with Delaware after posting identical 7–1 conference records.^[1][2] The Spiders earned a berth as the #8 seed into the 16-team Division I-AA playoffs, but lost in the quarterfinals to #1 seed Montana, 20–34.^[1] Richmond was led by sixth-year head coach Jim Reid.^[1]

The Spiders' win over Arkansas State in week four was their first against a Division I-A opponent since 1985.^[3]

Date	Time	Opponent	Rank	Site	Result	Attendance	Source
September 2	7:00 p.m.	Bucknell*		University of Richmond Stadium Richmond, VA	W 10–7	14,100
September 9	3:30 p.m.	at Virginia*		Scott Stadium Charlottesville, VA	L 6–34	50,285
September 16	1:00 p.m.	No. 3 UMass		University of Richmond Stadium Richmond, VA	W 31–24	10,200
September 30		at Arkansas State*	No. 19	Indian Stadium Jonesboro, AR	W 30–27	13,116
October 7	1:00 p.m.	No. 7 Delaware	No. 16	University of Richmond Stadium Richmond, VA	L 17–24	13,100
October 14	1:00 p.m.	at New Hampshire	No. 19	Wildcat Stadium Durham, NH	W 31–10	6,268
October 21		Maine	No. 19	University of Richmond Stadium Richmond, VA	W 17–6	10,075
October 28	1:00 p.m.	at No. 8 Villanova	No. 16	Villanova Stadium Villanova, PA	W 28–18	9,229
November 4	12:00 p.m.	at Rhode Island	No. 13	Meade Stadium Kingston, RI	W 13–10 OT	3,681
November 11	12:00 p.m.	No. 17 James Madison	No. 11	University of Richmond Stadium Richmond, VA (rivalry)	W 21–2	13,750	[4]
November 18	12:00 p.m.	at William & Mary	No. 10	Zable Stadium Williamsburg, VA (I-64 Bowl)	W 21–18	6,651
November 25	2:00 p.m.	No. 9 Youngstown State*	No. 10	University of Richmond Stadium Richmond, VA (NCAA Division I-AA First Round)	W 10–3	5,484
December 2	2:00 p.m.	at No. 1 Montana*	No. 10	Washington–Grizzly Stadium Missoula, MT (NCAA Division I-AA Quarterfinal)	L 20–34	17,345
*Non-conference game Homecoming Rankings from The Sports Network Poll released prior to the game All times are in Eastern time

• Second Team All-America – Eric Beatty (Associated Press, The Sports Network); Josh Spraker (Associated Press)
• First Team All-Atlantic 10 – Eric Beatty, Josh Spraker
• Second Team All-Atlantic 10 – Michael Millard, Mac Janney
• Third Team All-Atlantic 10 – Chad Blackstock, Harold Hill, Ken Farrar, David Lewandoski, Mark Thompson, TyRonne Turner
• Atlantic 10 Coach of the Year – Jim Reid