The 2000 Holy Cross Crusaders football team was an American football team that represented the College of the Holy Cross during the 2000 NCAA Division I-AA football season. Holy Cross finished second in the Patriot League.

In their fifth year under head coach Dan Allen, the Crusaders compiled a 7–4 record. David Puloka and Patrick Quay were the team captains.^[1]

The Crusaders outscored opponents 245 to 223. Their 4–2 conference record placed second in the seven-team Patriot League standings.^[2]

Holy Cross played its home games at Fitton Field on the college campus in Worcester, Massachusetts.

Date	Opponent	Site	Result	Attendance	Source
September 9	at Georgetown*	Kehoe Field Washington, DC	W 38–22	2,789	[3]
September 16	at Harvard*	Harvard Stadium Boston, MA	W 27–25	7,406	[4]
September 23	Towson	Fitton Field Worcester, MA	W 17–14	7,856	[5]
September 30	at Yale*	Yale Bowl New Haven, CT	L 27–33	22,509	[6]
October 7	Penn*	Fitton Field Worcester, MA	W 34–17	10,026	[7]
October 14	at Dartmouth*	Memorial Field Hanover, NH	L 14–31	5,014	[8]
October 21	at Lafayette	Fisher Field Easton, PA	L 13–28	6,723	[9]
October 28	No. 12 Lehigh^	Fitton Field Worcester, MA	L 6–21	9,228	[10]
November 4	Bucknell	Fitton Field Worcester, MA	W 10–9	6,147[11]	[1]
November 11	at Colgate	Andy Kerr Stadium Hamilton, NY	W 32–3	2,114	[12]
November 18	Fordham	Fitton Field Worcester, MA (rivalry)	W 27–20	9,147	[13]
*Non-conference game Homecoming ^ Family Weekend Rankings from The Sports Network Poll released prior to the game