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انهضام في الهلام.
الانهضام في الهلام (بالإنجليزية: In-gel digestion) هي خطوة في تحضير العينة لمطيافية الكتلة من أجل التعرف على البروتين وذلك في إطار الفحص البروتومي، وتتكون هذه الخطوة أساسا من أربعة مراحل: إزالة الصبغ، إختزال وألكلة السيستئينات في البروتين، القص المحلل للبروتين واستخلاص الببتيدات الناتجة.
تم تقديم هذه التقنية سنة 1992 من قبل روزنفيلد.[1] ورغم التغييرات العديدة والتحسينات التي طرأت عليها، إلى أن العناصر الأساسية لهذه العملية مازالت باقية.[2][3][4][5][6][7]
- ^ Rosenfeld, J et al., Anal Biochem, 1992, 203 (1), 173-9.
- ^ Hellman, U et al., Anal Biochem, 1995, 224 (1), 451-455.
- ^ Jeno, P et al., Anal Biochem, 1995, 224 (1), 75-82.
- ^ Shevchenko, A et al., Anal Chem, 1996, 68 (5), 850-8.
- ^ Borchers, C et al., Anal Chem, 2000, 72 (6), 1163-8.
- ^ Shevchenko, A et al., Nat Protoc, 2006, 1 (6), 2856-60.
- ^ Granvogl, B et al., Proteomics, 2007, 7 (5), 642-54.
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